AP Biology Premium, 2024 by Mary Wuerth
Author:Mary Wuerth
Language: eng
Format: epub
Publisher: Barrons Educational Services
Published: 2023-04-27T00:00:00+00:00
Long Free-Response
13.(a)POLR2A was used as the control because the expression of all the other genes was compared to the expression of POLR2A.
(b)POLR2A would be the best gene to use as a control because it is known to be expressed in all human tissues, as stated in the question.
(c)PDHA1 is most likely involved in glycolysis because glycolysis occurs in the cytoplasm of all living cells, and PDHA1 is expressed in all the tissues in the experiment.
(d)Since insulin is released when blood sugar levels are high, it is likely that the levels of insulin would be low when blood sugar levels are low. Therefore, when blood sugar levels are low, it is expected that both the INS mRNA and the insulin protein levels would be low.
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